HANNOVER MESSE 2019, 01 - 05 April
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Highly concentrated measuring of molecul

A microfluidic sample carrier for UV measurements.

Logo Karlsruher Institut für Technologie


Karlsruher Institut für Technologie

Exhibitor details
Exhibitor details
Logo Highly concentrated measuring of molecul

Product description

In biopharmaceutical process development, the concentration of protein and DNA samples is usually established with the aid of UV/Vis spectroscopy. The samples are examined using different wavelengths or spectral ranges. The amino acids or nucleobases contained in the samples absorb these light rays. Measured absorption ultimately correlates with the concentration of the sample examined. UV/Vis spectroscopy can be employed for further biotechnological applications such as colimetric assays. Micro-titre plates are widespread as sample carriers in day-to-day laboratory activities as well as in semi- or fully-automated process lines and robot platforms. However, the use of these platforms entails interference factors. Technology only allows the measurement of very low concentrations, so that samples have to be diluted and results extrapolated. A further disadvantage is that the surface tension of the dissolved samples causes a liquid meniscus to develop - the surface bends upwards towards its edge. The intensity of meniscus formation depends on the concentration of the sample. This results in inaccuracies when the measuring results are compared. KIT scientists have developed a silicon-based microfluidic sample carrier that eliminates the influence of diluting and menisci at reduced sample volume levels and thus provides qualitatively high-value measurement results. The (UV-) transparent sample carrier incorporates 96 closed sample channels with four measuring chambers each. The four chambers can be raised step by step, which enables measuring with four different thicknesses of layers in one single measurement process. Concentrations from 0.1 to 100 milligrams per millilitre can be measured. The design according to the 96 and 384 standard format allows the sample carriers to be used with established spectrophotometers - also with high throughputs. A casting process was applied to make the new sample carriers at the Institute.

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Hall 2, Stand B16

(Main stand)

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